MOTS-c during seven days of rat hindlimb unloading
D. A. Sidorenko, I. D. Lvova, S. A. Tyganov, B. S. Shenkman, K. A. Sharlo · 2025 · J Muscle Res Cell Motil. 46(3):215–229; PMID 40608240
Published 2025-07-03.
Related evidence maps: Sports performance ↗ · Military & occupational ↗
Reported findings and study design
MOTS-c preserved isolated soleus fatigue resistance and mitigated slow-fiber atrophy. Fast-fiber atrophy was not mitigated.
Male Wistar rats undergoing seven days of hindlimb suspension; final fatigue test in excised soleus muscle
Protocol information
Daily intraperitoneal injections during seven days of unloading. Dose and group-level denominators are not given in the verified abstract.
Protocol source ↗ · Publisher abstract and PubMed record checked; subscription PDF, numerical figures and complete treatment methods not recovered
| Measure | Result | Comparison, timing and statistics |
|---|---|---|
| Ex vivo soleus fatigue | Increase in fatigue with unloading was prevented in the treated group | After 7 days; numerical effect size unavailable in the abstract. |
| Fiber-type changes | Attenuated slow-fiber atrophy and slow-to-fast shift; fast-fiber atrophy not prevented | Histology; absolute cross-sectional areas not verified. |
| Signaling and ribosomal RNA | Preserved Akt/GSK3β phosphorylation and 18S/28S rRNA near control levels | Biochemical measures in rat soleus. |
| Atrophy and mitochondrial markers | Lower MuRF and Atrogin-1 mRNA; preservation of selected biogenesis markers and ACC phosphorylation | Compared with unloaded placebo animals; no causal mediation established. |
A muscle-disuse preservation experiment, relevant to space medicine and immobilization. The abstract does not establish human protection or an ergogenic effect.
Source for the extracted data ↗ · Checked 2026-11-08
Molekul summary of the linked publication. Educational information, not medical advice. Seek qualified healthcare help for medical conditions.
Methods, limitations & source review
Study limitations
- Original numerical fatigue effect and group sizes require full text.
- Ex vivo muscle function after disuse differs from athletic endurance in an exercising person.
- Several molecular readouts do not establish one causal pathway.
Publication counts are not counts of independent trials. Reviews, translations and reports sharing participants may overlap. Findings apply to the studied preparation, population and endpoints.
Document format: Publisher abstract and PubMed record checked; subscription PDF, numerical figures and complete treatment methods not recovered
Review depth: Reported methods, results and evidence limitations
Source access and review scope
Publisher abstract and PubMed record checked; subscription PDF, numerical figures and complete treatment methods not recovered
Recorded source-check date: 2026-11-08. This date does not imply a completed systematic review.
Source type: Original journal article
Citation and original source
Original source: D. A. Sidorenko, I. D. Lvova, S. A. Tyganov, B. S. Shenkman, K. A. Sharlo. MOTS-c during seven days of rat hindlimb unloading J Muscle Res Cell Motil. 46(3):215–229; PMID 40608240 2025.
Molekul research summary: https://molekul.io/research/mots-c-soleus-unloading-2025. Cite the original publication for its findings and this page when referring to Molekul’s summary or evidence appraisal.
Bibliography entry