Cortexin and Semax in a prolonged rat cold-stress model
V. P. Ganapolsky, P. V. Agafonov, V. O. Matytsyn · 2022 · Pediatrician 13(6):85–96
Indexed 2026-11-09.
Related evidence maps: Military & occupational ↗
Reported findings and study design
Day-14 swim time was 3.2±0.1 min with Semax and 1.3±0.1 min with either saline or Cortexin. The 35°C plateau lasted 10, 12 and 14 days across the three groups.
Ninety outbred male rats, 30 per group. Prolonged cold exposure combined with altered lighting, noise and dietary restriction. Serial thermoregulation and weighted-swim measurements.
Protocol information
Methods specify intraperitoneal Cortexin 5 mg/kg and Semax 0.3 mg/kg twice daily, beginning three days before cold stress. The discussion specifies subcutaneous administration and the conclusion says once daily. The route and frequency conflict.
Protocol source ↗ · Original methods and Table 5
| Measure | Result | Comparison, timing and statistics |
|---|---|---|
| Semax · day-14 limiting swim | 3.2±0.1 vs 1.3±0.1 min saline | Table 5, p<0.05 versus saline as marked. |
| Cortexin · day-14 limiting swim | 1.3±0.1 vs 1.3±0.1 min saline | Earlier differences did not persist at day 14. |
| Temperature plateau near 35°C | 10 days saline; 12 Cortexin; 14 Semax | Reported thermoregulation endpoint, separate from the swim outcome. |
| Publication overlap | Identical weighted-swim means and uncertainties at all six time points | The 2023 Russian Biomedical Research paper (DOI 10.56871/RBR.2023.20.34.002) repeats Table 5. Count one animal experiment. |
Table 5 · maximum weighted swim time during cold stress
| Day | Placebo (min) | Cortexin (min) | Semax (min) |
|---|---|---|---|
| 0 | 9.5±0.8 | 9.8±0.7 | 9.4±0.6 |
| 5 | 8.4±1.2 | 8.5±0.9 | 9.5±1.2 |
| 7 | 5.2±0.6* | 7.0±0.5# | 7.9±0.8# |
| 10 | 3.2±0.3* | 4.9±0.3# | 5.2±0.4# |
| 12 | 3.1±0.5* | 3.6±0.7 | 5.4±0.6# |
| 14 | 1.3±0.1* | 1.3±0.1 | 3.2±0.1# |
M±m. * p<0.05 versus day 0, # p<0.05 versus placebo as printed. Both publications print the same values.
Cortexin and saline had the same mean swim time at day 14. The paper reports a 14-day temperature plateau for Semax.
Source for the extracted data ↗ · Checked 2026-11-09
Molekul summary of the linked publication. Educational information, not medical advice. Seek qualified healthcare help for medical conditions.
Methods, limitations & source review
Study limitations
- The report conflicts on administration route and frequency. Cortexin and Semax were given to separate groups.
- The 2023 report repeats the weighted-swim table. It supplies no independent animal count or human performance evidence.
Publication counts are not counts of independent trials. Reviews, translations and reports sharing participants may overlap. Findings apply to the studied preparation, population and endpoints.
Document format: Original methods and Table 5
Review depth: Reported methods, results and evidence limitations
Related-report group: ganapolsky-cold-stress-2022-2023. Reports in this group are not assumed to be independent trials.
Source access and review scope
Original methods and Table 5
Recorded source-check date: 2026-11-09. This date does not imply a completed systematic review.
Source type: Pediatrician 13(6):85–96
Open original source or index record ↗
DOI: 10.17816/PED13685-96
Citation and original source
Original source: V. P. Ganapolsky, P. V. Agafonov, V. O. Matytsyn. Cortexin and Semax in a prolonged rat cold-stress model Pediatrician 13(6):85–96 2022.
Molekul research summary: https://molekul.io/research/ganapolsky-cortexin-semax-cold-stress-2022. Cite the original publication for its findings and this page when referring to Molekul’s summary or evidence appraisal.
Bibliography entryRelated compound profiles
Cortexin
A tissue-derived preparation with Russian stroke, cognitive, pediatric and other clinical reports. Study designs vary; the independent stroke review is shown alongside favorable original reports. None validates the personal WHOOP diary.
Semax
ACTH-fragment-derived peptide with clinical stroke and optic-nerve reports, healthy-volunteer imaging and preclinical neuroregulation research. Precursor, patent and dissertation sources remain separately labeled.