Effects of vitamin B1/B6/B12 complex on methotrexate-induced hepatorenal toxicity in a short-term acute rat model
Toprak-Semiz A, Deniz E, Topçu A, Mataracı Karakaş S, Öztürk Ç, Yavuz-Bedir E · 2026 · Naunyn-Schmiedeberg's archives of pharmacology
Published 2026-10-13. Indexed 2026-10-19.
Reported findings and study design
B1/B6/B12 combinations improved selected biochemical measures after methotrexate exposure. Both doses lowered creatinine and renal TBARS; only the higher dose lowered AST and hepatic TBARS. Neither dose significantly improved liver or kidney histopathology, ALT, urea or total thiol.
Dose, route and schedule
B1/B6/B12 intraperitoneally daily on days 1–14 at 16/16/0.16 or 32/32/0.32 mg/kg; methotrexate 20 mg/kg intraperitoneally once on day 7. Rat study methods; formulation not established as Binavit.
Protocol source ↗ · Indexed abstract checked
| Measure | Result | Comparison, timing and statistics |
|---|---|---|
| Low vitamin dose | B1/B6/B12: 16/16/0.16 mg/kg daily | Intraperitoneal, days 1–14; animal doses |
| High vitamin dose | B1/B6/B12: 32/32/0.32 mg/kg daily | Intraperitoneal, days 1–14; animal doses |
| Creatinine and renal TBARS | Lower at both vitamin doses | Versus methotrexate alone |
| AST and hepatic TBARS | Lower only at the higher dose | |
| Histopathology, ALT, urea and total thiol | No significant improvement |
This is class-level B-vitamin research in rats, not evidence for the Binavit brand or human methotrexate care. No vitamin-only group was included, and biochemical changes were not accompanied by significant tissue-level improvement.
Source for the extracted data ↗ · Checked 2026-10-31
Molekul summary of the linked publication. Educational information, not medical advice. Seek qualified healthcare help for medical conditions.
Methods, limitations & source review
Study limitations
- This is class-level B-vitamin research in rats, not evidence for the Binavit brand or human methotrexate care. No vitamin-only group was included, and biochemical changes were not accompanied by significant tissue-level improvement.
Publication counts are not counts of independent trials. Reviews, translations and reports sharing participants may overlap. Findings apply to the studied preparation, population and endpoints.
Review depth: Indexed abstract checked
Source access and review scope
Primary publication metadata and indexed abstract checked through Europe PMC on 31 October 2026. No unavailable effect sizes inferred.
Recorded source-check date: 2026-10-31. This date does not imply a completed systematic review.
Source type: Journal publication
Citation and original source
Original source: Toprak-Semiz A, Deniz E, Topçu A, Mataracı Karakaş S, Öztürk Ç, Yavuz-Bedir E. Effects of vitamin B1/B6/B12 complex on methotrexate-induced hepatorenal toxicity in a short-term acute rat model Naunyn-Schmiedeberg's archives of pharmacology 2026.
Molekul research summary: https://molekul.io/research/oct31-42864503. Cite the original publication for its findings and this page when referring to Molekul’s summary or evidence appraisal.
Bibliography entry